4. Features Of Immunohistochemical Changes In Proximal And Distal Renal Tubules In Patients Died From Sepsis
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Abstract
Materials and methods. Sectional materials were studied in 5 patients who died from sepsis: 4 women (80%) and 1 man (20%). Histological structural changes in renal tissue were investigated by histochemical and immunohistochemical methods. The autopsy was performed at an early stage (1-6 hours after death). The control group has included 10 kidneys of healthy people who died, as a result, an accident. Sections were made from paraffin blocks. Sections 6 μ thick were cut from paraffin blocks and Anty-E. Coli LPS anti-body (2D7/1) 1/100 by the immunocytochemical method was used..
Results. Pronounced changes were observed in the proximal, distal tubules of the kidneys and Henle loop cells in histochemical and immunochemical studies. The following 1 (20%) patients had 3 (+ + +) stain-ing in the proximal tubules, 2 (40%) 2 (++) in the proximal and distal tubules, 1 (20%) 1 (+) weak staining. Staining was not observed in 1 (20%) patient..
Conclusion. These changes were manifested primarily as a result of the accumulation of lymphocytes in the area of inflammation. Thus, under the influence of endotoxins LPS (lipopolysaccharide) with bacterial intoxication, irreversible changes in epithelial cells and sensitive tubules are not rarely observed, accom-panied by fragmentation of the microvilli of the brush border on the apical part of the epithelial cells of the proximal tubules.
Keywords
sepsis, LPS endotoxin
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