3. Prospects for the use of PCR in the detection of hepatitis (literature review)

Authors

  • А.Т. Аубакирова «Syzganov National Scientific Center of Surgery» JSC, the Republic of Kazakhstan;
  • Л.Е. Ризабекова «Syzganov National Scientific Center of Surgery» JSC, the Republic of Kazakhstan;
  • Г.Б. Абдилова «Syzganov National Scientific Center of Surgery» JSC, the Republic of Kazakhstan;
  • . RNA. «Syzganov National Scientific Center of Surgery» JSC, the Republic of Kazakhstan;

DOI:

https://doi.org/10.35805/BSK2023IV003

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Abstract

Relevance: According to official WHO data, currently, about 325 million people in the world live with chronic hepatitis B and C, which makes hepatitis one of the most common infectious diseases in the world. There are many scientific studies aimed at a deeper understanding of hepatitis and the development of new methods of treatment and diagnosis. With the help of polymerase chain reaction, more sensitive tests for diagnosing hepatitis have been developed, allowing faster infection detection and treatment initiation. The PCR method for diagnosing hepatitis is justified and relevant, as it is one of the most effective and modern methods for detecting viral infection, which allows you to start treatment earlier and prevent the development of complications. The study aimed to evaluate the PCR method’s efficiency in diagnosing hepatitis.

Methods: In this review, the causes of hepatitis were investigated, and a comparative assessment of the effectiveness of various laboratory methods for diagnosing hepatitis, including enzyme immunoassay and polymerase chain reaction, was conducted. The analysis of scientific publications revealed the factors associated with the development of hepatitis and compared the effectiveness of ELISA, used for serological analysis of antibodies to hepatitis viruses, with PCR, designed to detect DNA or RNA of hepatitis viruses. The obtained results provide important information for improving the methods of diagnosis of hepatitis and more effective control of this serious infectious pathology.

Results: The analyzed scientific literature has shown that PCR diagnostics has several advantages over other methods of hepatitis diagnosis, such as ELISA, including higher sensitivity and specificity, the ability to detect hidden infections, and faster. results. In addition, PCR diagnostics can be used to determine a specific strain of the virus, making it possible to take measures to treat and control infection. All these advantages confirm the scientific validity of using the PCR method in detecting hepatitis.

Conclusion: According to global statistics, PCR has a higher sensitivity and specificity in diagnosing hepatitis than ELISA. PCR can also be used to monitor the effectiveness of hepatitis treatment and determine viral load to make decisions about the need for treatment and evaluate its effectiveness.

Keywords

hepatitis, enzyme immunoassay, polymerase chain reaction, sensitivity, detection, DNA, RNA

References

  1. Zauli D.A., Menezes C.L., Oliveira C.L., Mateo E.C., Ferreira A.C. In-house quantitative real-time PCR for the diagnosis of hepatitis B virus and hepatitis C virus infections. Braz J Microbiol. 2016;47(4):987-992. doi:10.1016/j.bjm.2016.07.008
  2. Taylor M., Alonso-González M., Gómez B., Korenromp E., Broutet N. World Health Organization global health sector strategy on sexually transmitted infections: an evidence-to-action summary for Colombia. Revista colombiana de obstetricia y ginecologia. 2017;68(3):193-201
  3. PCR diagnostics can detect the presence of the virus directly, while ELISA diagnostics only detects the presence of antibodies to the virus. This means that PCR diagnostics can be used to determine the presence of the virus in blood, tissues, and other biological materials
  4. Kozak R.A., Rutherford C., Richard-Greenblatt M., et al. Development and Evaluation of a Molecular Hepatitis A Virus Assay for Serum and Stool Specimens. Viruses. Jan 15 2022;14(1) doi:10.3390/v14010159
  5. Park Y., Kim B.S., Choi K.H., et al. A novel multiplex real-time PCR assay for the concurrent detection of hepatitis A, B and C viruses in patients with acute hepatitis. PLoS One. 2012;7(11):e49106. doi:10.1371/journal.pone.0049106
  6. Mohd Hanafiah K., Groeger J., Flaxman A.D., Wiersma S.T. Global epidemiology of hepatitis C virus infection: new estimates of age-specific antibody to HCV seroprevalence. Hepatology. Apr 2013;57(4):1333-42. doi:10.1002/hep.26141
  7. Easterbrook P.J., Roberts T., Sands A., Peeling R. Diagnosis of viral hepatitis. Curr Opin HIV AIDS. May 2017;12(3):302-314. doi:10.1097/COH.0000000000000370ProspectsfortheuseofPCRinthedetectionofhepatitis(aliteraturereview)
  8. Davydov V., Babenko A., Zhavoronok S., Marchuk S., Borisovec D., Znovec T. Novaja test-sistema dlja vyjavlenija RNK virusa gepatita E metodom PCR v rezhime real’nogo vremeni. Laboratornaja diagnostika Vostochnaja Evropa. 2021;10(3):346-359
  9. Prevention CfDCa. Hepatitis C questions and answers for health professionals. Retrieved February. 2020;25:2020
  10. Hemminki K. Immigrant health, our health. Eur J Public Health. Aug 2014;24 Suppl 1:92-5. doi:10.1093/eurpub/cku108
  11. Cuomo G., Franconi I., Riva N., et al. Migration and health: A retrospective study about the prevalence of HBV, HIV, HCV, tuberculosis and syphilis infections amongst newly arrived migrants screened at the Infectious Diseases Unit of Modena, Italy. J Infect Public Health. 2019;12(2):200-204. doi:10.1016/j.jiph.2018.10.004
  12. Guidelines for the Screening Care and Treatment of Persons with Chronic Hepatitis C Infection: Updated Version. 2016
  13. Mazzeo C., Azzaroli F., Giovanelli S., et al. Ten year incidence of HCV infection in northern Italy and frequency of spontaneous viral clearance. Gut. Jul 2003;52(7):1030-4. doi:10.1136/gut.52.7.1030
  14. Raviglione M., Sulis G. Tuberculosis 2015: Burden, Challenges and Strategy for Control and Elimination. Infect Dis Rep. Jun 24 2016;8(2):6570. doi:10.4081/idr.2016.6570
  15. Sulis G., Roggi A., Matteelli A., Raviglione M.C. Tuberculosis: epidemiology and control. Mediterr J Hematol Infect Dis. 2014;6(1):e2014070. doi:10.4084/MJHID.2014.070
  16. McNeil C.J., Bachmann L.H. Syphilis: An Old Disease With Present-Day Implications. N C Med J. 2016;77(5):365-8. doi:10.18043/ncm.77.5.365
  17. Chevaliez S. Virological tools to diagnose and monitor hepatitis C virus infection. Clin Microbiol Infect. Feb 2011;17(2):116-21. doi:10.1111/j.1469-0691.2010.03418.xProspectsfortheuseofPCRinthedetectionofhepatitis(aliteraturereview)

Published

2023-12-22

Issue

No. 4 (2023)

Section

Статьи