2. Опыт применения аппарат «Аклидес» в Казахстане для диагностики системных аутоиммунных заболеваний

Authors

  • A.D. Nurakhova Kazakh Medical University of Continuing Education, Almaty, Kazakhstan
  • A.M. Maimakova Kazakh Medical University of Continuing Education, Almaty, Kazakhstan
  • G.B. Abdilova National Scientific Surgery Center under the name of A.N.Syzganov, Almaty, Kazakhstan
  • G.K. Dalibaeva Medical center “Asmed”, Almaty, Kazakhstan

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Abstract

The main methods of laboratory diagnosis of autoimmune diseases (AD) are: the reaction of indirect immu-nofluorescence, enzyme immunodetection and immunoblotting. There are direct and indirect reaction of immu-nofluorescence. The method of direct immunofluorescence is used to detect Ig deposits and complement factors in biopsy samples of skin and kidneys. The reaction of indirect immunofluorescence is used to detect Ab in the blood serum and other biological fluids. It should be noted that this method requires very high qualification of the technician. The disadvantages of this method are the subjective account of the results and the complexity, lack of standardization of substrates, reagents, microscope. Computer-based systems for interpreting cell fluorescence tests contribute to the standardization and improvement of reproducibility of the reaction of indirect immunofluo-rescence in the determination of autoantibodies in patients with rheumatoid diseases. named after A. N. Syzganova medical sciences The blood serum of 17 patients were studied with this method for the presence of the following autoan-tibodies: Antinuclear Antibodies (ANA), Cytoplasmic antineutrophil antibodies (cANCA), Perinuclear Anti-neutrophil Antibodies (pANCA). The studies were conducted on the AKLIDES apparatus made by company “Medipan GmbH”, Germany. Modern methods of serological diagnosis RIF were applied in Kazakhstan for the first time to assess serological activity of SLE. Currently in Kazakhstan there is a need for accurate diag-nostics of autoimmune rheumatic diseases. The correct diagnosis with early stages of this disease will stop the process of disease progression and improve life quality of patients.

Keywords

autoimmune diseases, laboratory diagnosis, indirect immunofluo-rescence reaction, autoimmune antibodies, immunoblot

References

  1. Laboratory autoimmune diagnostics from a purely scientific investigation method is trans-forming into an independent section of clinical laboratory diagnostics
  2. The current state of knowledge and techno-logical innovations, particularly in the area of digital analysis of immunofluorescence imag-es, allow to provide the necessary standard-ization and harmonization of autoantibodies detection results
  3. Although the use of software and hardware complexes is still relatively expensive and, fur-thermore, requires detailed exploration by the laboratory staff, however, their use leads to rapid development of ARD diagnostics by ob-taining accurate and standardized results, the quality of which can compete with the quality of expert laboratories. Note: *, 0 – pop-up values of the variational series. Figure
  4. For the first time in Kazakhstan a survey on the apparatus AKLIDES was performed to deter-mine autoimmune antibodies in order to clarify the diagnoses of the patients
  5. New system AKLIDES provides the automatic reading of the images of IIF and includes soft-ware algorithms for the mathematical descrip-tion of the indirect immunofluorescence of autoantibodies picture. THE EXPERIENCE OF APPLICATION OF APPARATUS “AKLIDES” IN KAZAKHSTAN FOR THE DIAGNOSIS OF SYSTEMIC AUTOIMMUNE DISEASES
  6. Kumar Y, Bhatia A, Minz RW. Antinuclear antibodies and their detection methods in diagnosis of con-nective tissue diseases: a journey revisited. Diagn Pathol. 2009 Jan 2;4:1. PMID: 19121207 [PubMed]
  7. Mariz HA, Sato EI, Barbosa SH, Rodrigues SH, Del-lavance A, Andrade LE. Pattern on the antinuclear antibody-HEp-2 test is a critical parameter for dis-criminating antinuclear antibody-positive healthy individuals and patients with autoimmune rheumatic diseases. Arthritis Rheum. 2011 Jan;63(1):191-200
  8. Moore AE, Sabachewsk L, Toolan HW: Culture char-acteristics of four permanent lines of human cells. Cancer Res. 1955, 15:998-602
  9. Tan EM: Antibodies to nuclear antigens (ANA) and their immunobiology and medicine. Adv Immunol. 1982, 33:167-240
  10. Tan EM: Antinuclear antibodies: Diagnostic markers for autoimmune diseases and probes for cell biology. Adv Immunol. 1989, 44:93-151
  11. Weller TH, Coons AH: Fluorescent antibody studies with agents of Varicella and Herpes zoster propagat-ed in vitro. Proc Soc Exp Biol Med 1954, 86:789-794
  12. Wilson D. McGraw-Hill Manual of Laboratory and Di-agnostic Tests 1st Ed Normal, Illinois, 2007: 55-57 pp
  13. Sozina А.V.N.U.А., Tikhomirova Т.А., Lapin S.V. Com-bined incidence of autoantibodies in patients with diffuse connective tissue diseases // Medical immu-nology. -
  14. Wiik, A., Cervera, R., Haass, M., Kallenberg, C., Khamashta, M., Meroni, P. L., Piette, J. C., Schmitt, R., Shoenfeld, Y. European attempts to set guidelines for improving diagnostics of autoimmune rheumatic disorders // Lupus. -
  15. Damoiseaux J. Autoantibodies in the grocery shop: does quantity matter? // Immunol Res. -
  16. Hiemann, R., Buttner, T., Krieger, T., Roggenbuck, D., Sack, U., Conrad, K.Challenges of automated screening and differentiation of non-organ specific autoantibodies on HEp-2 cells // Autoimmun Rev. -2009. Vol.9, N1. P.17-22. 14
  17. Tozzoli R A.A., Porcelli B, Bassetti D. . Automation in indirect immunofluorescense testing: a new step in the evolution of the autoimmunology laboratory. // Autoimmun Highlights. -
  18. Damoiseaux J. Autoantibodies in the grocery shop: does quantity matter? // Immunol Res. -
  19. Hiemann, R., Buttner, T., Krieger, T., Roggenbuck, D., Sack, U., Conrad, K.Challenges of automated screening and differentiation of non-organ specific autoantibodies on HEp-2 cells // Autoimmun Rev. -2009. Vol.9, N1. P.17-22. 14
  20. Tozzoli R A.A., Porcelli B, Bassetti D. . Automation in indirect immunofluorescense testing: a new step in the evolution of the autoimmunology laboratory. // Autoimmun Highlights. -
  21. Hiemann, R., Hilger, N., Michel, J., Nitschke, J., Bohm, A., Anderer, U., Weigert, M., Sack, U. Au-tomatic analysis of immunofluorescence patterns of HEp-2 cells // Ann N Y Acad Sci. -
  22. Bossuyt, X., Cooreman, S., De Baere, H., Ver-schueren, P., Westhovens, R., Blockmans, D., Marien, G. Detection of antinuclear antibodies by automated indirect immunofluorescence analysis // Clin Chim Acta. -
  23. Kivity, S., Gilburd, B., Agmon-Levin, N., Carrasco, M. G., Tzafrir, Y., Sofer, Y., Mandel, M., Buttner, T., Roggenbuck, D., Matucci-Cerinic, M., Danko, K., Hoyos, M. L., Shoenfeld, Y. A novel automated indi-rect immunofluorescence autoantibody evaluation // Clin Rheumatol. -
  24. Willitzki, A., Lorenz, S., Hiemann, R., Guttek, K., Goihl, A., Hartig, R., Conrad, K., Feist, E., Sack, U., Schierack, P., Heiserich, L., Eberle, C., Peters, V., Roggenbuck, D., Reinhold, D. Fully automated anal-ysis of chemically induced gammaH2AX foci in hu-man peripheral blood mononuclear cells by indirect immunofluorescence // Cytometry A. -
  25. Roggenbuck, D., Hiemann, R., Bogdanos, D., Rein-hold, D., Conrad, K. Standardization of automated interpretation of immunofluorescence tests // Clin Chim Acta. -
  26. Roggenbuck, D., Reinhold, D., Hiemann, R., Ander-er, U., Conrad, K.Standardized detection of anti-ds DNA antibodies by indirect immunofluorescence - a new age for confirmatory tests in SLE diagnostics // Clin Chim Acta. -
  27. Conrad K, Sack U. Multiparametric analyses in diag-nostics and monitoring of autoimmune diseases: cur-rent state and perspectives. // Laboratoriumsmed-izin-Journal of Laboratory Medicine. -
  28. Egerer, K., Roggenbuck, D., Hiemann, R., Weyer, M. G., Buttner, T., Radau, B., Krause, R., Lehmann, B., Feist, E., Burmester, G. R. Automated evaluation of autoantibodies on human epithelial-2 cells as an approach to standardize cell-based immunofluores-cence tests // Arthritis Res Ther. -
  29. Knutter, I., Hiemann, R., Brumma, T., Buttner, T., Grossmann, K., Cusini, M., Pregnolato, F., Borghi, M. O., Anderer, U., Conrad, K., Reinhold, D., Roggen-buck, D., Csernok, E.Automated interpretation of ANCA patterns - a new approach in the serology of ANCA-associated vasculitis // Arthritis Res Ther. -2012. Vol.14, N6. P.R271
  30. Czaja A.J., Freese D.K. Diagnosis and treatment of autoimmune hepatitis // Hepatology. -
  31. Meroni, P. L., Borghi, M. O., Raschi, E., Tedesco, F. Pathogenesis of antiphospholipid syndrome: un-derstanding the antibodies // Nat Rev Rheumatol. -2011. Vol.7, N6. P.330-339
  32. Savige, J., Davies, D., Falk, R. J., Jennette, J. C., Wiik, A. Antineutrophil cytoplasmic antibodies and associated diseases: a review of the clinical and laboratory features // Kidney Int. -
  33. Lapin S.V., Totolian A.A. Immunolgical diagnostics of kidney involvment in vasculitis // Nephrologia -
  34. Wiik A. Autoantibodies in vasculitis // Arthritis Res Ther. -
  35. Togizbaev H.A. Diagnosis of systemic autoimmune diseases // Man and Medicine – Kazakhstan – 2012.-№8 (12).- p. 144-145

Published

2019-10-01

Issue

No. 3 (2019)

Section

Статьи